Part:BBa_K1510931:Design

Sequence integrated the luxR gene and pluxR promoter together.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 10
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1019
  

Design Notes

We slightly alter the former arrangement of the V.fischeri AHL-LuxR quorum sensing system to make the pluxR promoter lie below the luxR gene in order to carry on the whole quorum sensing pathway in an align.

Source

BBa_K1510000 and BBa_R0062.